纳米壳聚糖复合材料的制备与性能研究 -

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纳米壳聚糖复合材料的制备与性能研究

doi:10.3788/CO.20181105.0773

董丽丹^{1, 2,},
魏长平^1,,,
李中田³,
汪凤明¹

1.
长春理工大学材料科学与工程学院, 吉林长春 130022
2.
长春医学高等专科学校, 吉林长春 130031
3.
中水东北勘测设计研究有限责任公司, 吉林长春 130061

基金项目:

中国科协"海外智力为国服务行动计划"项目07064

吉林省教育厅"十二五"科学技术研究项目【2013】36

详细信息

作者简介:
董丽丹(1980-), 女, 吉林长春人, 硕士, 讲师, 2007年于长春理工大学获得硕士学位, 现为长春医学高等专科学校讲师、长春理工大学在读博士研究生, 主要从事医用壳聚糖材料方面的研究。E-mail:573972433@qq.com
魏长平(1960—)，女，吉林长春人，教授，博士生导师，1999年于吉林大学获得博士学位，主要从事医用基础功能材料的研究。E-mail:changpingwei@hotmail.com

中图分类号:TP394.1;TH691.9
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- 被引次数:0
出版历程
- 收稿日期:2018-04-09
- 修回日期:2018-05-15
- 刊出日期:2018-10-01

Preparation and properties of nano-chitosan composites

1.
School of Materials Science and Engineering, Changchun University of Science and Tech., Changchun 130022, China
2.
School of drug and food, Changchun Medical College, Changchun 130031, China
3.
China Water Northeastern Investigation, Design and Research Co., Ltd, Changchun 130061, China

Funds:

China Association for Science and Technology "Overseas Intelligence Service Action Plan"07064

The Education Department of Jilin Provincial "The 12th Five-year Scientific and Technological Research Project"【2013】36

More Information

Corresponding author:WEI Chang-ping, E-mail:changpingwei@hotmail.com

摘要

摘要:为了提高壳聚糖的水溶性及其止血方面的性能，将壳聚糖（CS）纳米化，并引入具有抗菌作用的Ag ⁺离子和凝血辅助作用的Ca ²⁺离子，制备出纳米壳聚糖金属离子复合止血材料。首先，采用离子交换法制备纳米壳聚糖（nmCS），再分别加入AgNO ₃和饱和CaCl ₂溶液，制得nmCS-Ag、nmCS-Ca、nmCS-Ag-Ca复合材料。然后，采用FTIR、XRD、SEM等手段对复合材料的结构进行表征。最后，对复合物的凝血、止血性能进行了测试。实验结果表明：改性后的壳聚糖IR图谱在1 647 cm ^-1和1 560 cm ^-1处出现了纳米壳聚糖钠盐的特征吸收峰；复合了金属离子的纳米壳聚糖在XRD图谱中表现出了Ag ⁺、Ca ²⁺的晶型特征；扫描电镜显示nmCS-Ag中Ag ⁺有部分析出而nmCS-Ca的复合效果较好；nmCS-Ag-Ca的凝血、止血效果要优于nmCS-Ag和nmCS-Ca，同时nmCS-Ag和nmCS-Ca的凝血、止血效果要优于nmCS。测试结果表明，成功制备了纳米壳聚糖金属离子复合止血材料。
- 壳聚糖/
- 复合材料/
- 纳米/
- 性能
Abstract:In order to improve the water soluble and the hemostatic performance of chitosan (CS), chitosan is nano-sized and an antibacterial Ag ⁺and coagulation-assisted Ca ²⁺are introduced to prepare a nano-chitosan metal ion composite hemostatic material. Firstly, nano-chitosan (nmCS) is prepared by ion exchange method, and AgNO ₃and saturated CaCl ₂solution are added respectively to obtain nmCS-Ag, nmCS-Ca and nmCS-Ag-Ca composites. Then, the structure of the composite is characterized by means of FTIR, XRD and SEM. Finally, the coagulation and hemostatic properties of the complex are tested. The results show that the characteristic absorption peak of nano-chitosan sodium salt appeared at the modified chitosan IR spectrum at 1 647 cm ^-1and 1 560 cm ^-1. The nano-chitosan composited with metal ions show the crystal characteristics of Ag ⁺and Ca ²⁺in the XRD pattern. The SEM shows that partial Ag ⁺precipitates in nmCS-Ag, and the composite effect of nmCS-Ca is better. The coagulation and hemostasis of nmCS-Ag-Ca is better than that of nmCS-Ag and nmCS-Ca, while the coagulation and hemostasis of nmCS-Ag and nmCS-Ca are better than that of nmCS. The test results show that the nano-chitosan metal ion composite hemostatic material has been successfully prepared.
- nano-chitosan/
- composite materials/
- characterization/
- performance

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图 1CS和nmCS的红外光谱图

Figure 1.FT-IR spectra of chitosan(a) and nm chitosan(b)

下载: 全尺寸图片幻灯片

图 2nmCS复合Ag⁺、Ca²⁺的红外光谱图

Figure 2.FT-IR spectra of nm Chitosan composite Ag⁺, Ca²⁺

下载: 全尺寸图片幻灯片

图 3CS和nmCS的XRD图

Figure 3.XRD spectra of chitosan(a) and nm chitosan(b)

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图 4nmCS复合Ag⁺、Ca²⁺的XRD图

Figure 4.XRD spectra of nm Chitosan composite Ag⁺, Ca²⁺

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图 5nmCS、Ca-nmCS及Ag-nmCS扫描电镜图

Figure 5.SEM micrographs of nm Chitosan composite Ag⁺、Ca²⁺

下载: 全尺寸图片幻灯片

表 1样品的凝血时间测试

Table 1.Clotting time test of the sample

样品	编号	凝血时间	平均凝血时间
自然凝血	1	5′37″	5′18″
	2	5′2″
	3	5′16″
CS	1	3′40″	3′56″
	2	3′23″
	3	3′40″
nmCS	1	3′23″	3′27″
	2	3′20″
	3	3′37″
Ag⁺-nmCS	1	3′7″	3′17″
	2	3′25″
	3	3′19″
Ca²⁺-nmCS	1	3′22″	3′13″
	2	3′14″
	3	3′3″
Ag⁺-Ca²⁺-nmCS	1	2′55″	3′2″
	2	3′7″
	3	3′5″

下载: 导出CSV

表 2样品的止血时间测试

Table 2.Bleeding time test of the sample

样品	编号	止血时间	平均止血时间
自然止血	1	18′37″	17′34″
	2	16′24″
	3	17′40″
CS	1	9′25″	9′46″
	2	10′5″
	3	9′48″	9′24″
nmCS	1	9′40″
	2	9′8″
	3	9′25″
Ag⁺- nmCS	1	8′40″	8′37″
	2	8′23″
	3	8′49″
Ca²⁺-nmCS	1	8′28″	8′9″
	2	7′45″
	3	8′13″
Ag⁺-Ca²⁺-nmCS	1	7′36″	7′12″
	2	6′46″
	3	7′15″

下载: 导出CSV

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