Dec. 2022

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Chinese Optics> 2022> 15(6): 1167-1181.

CHEN Shuai, REN Lin, ZHOU Zhen-qiao, LI Min, JIA Hong-bo. In-vivo across-scales two-photon microscopic imaging technique[J]. Chinese Optics, 2022, 15(6): 1167-1181. doi: 10.37188/CO.2022-0086

Citation:

CHEN Shuai, REN Lin, ZHOU Zhen-qiao, LI Min, JIA Hong-bo. In-vivo across-scales two-photon microscopic imaging technique[J].Chinese Optics, 2022, 15(6): 1167-1181.doi:10.37188/CO.2022-0086

CHEN Shuai, REN Lin, ZHOU Zhen-qiao, LI Min, JIA Hong-bo. In-vivo across-scales two-photon microscopic imaging technique[J]. Chinese Optics, 2022, 15(6): 1167-1181. doi: 10.37188/CO.2022-0086

Citation:

CHEN Shuai, REN Lin, ZHOU Zhen-qiao, LI Min, JIA Hong-bo. In-vivo across-scales two-photon microscopic imaging technique[J].Chinese Optics, 2022, 15(6): 1167-1181.doi:10.37188/CO.2022-0086

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In-vivo across-scales two-photon microscopic imaging technique

doi:10.37188/CO.2022-0086

CHEN Shuai^{1, 2,},
REN Lin^{1, 2,},
ZHOU Zhen-qiao^2,,
LI Min^2,,
JIA Hong-bo^{1, 2,,}

1.
School of Physical Science and Engineering Technology and Center for Brain and Intelligence Research, Guangxi University, Nanning 530004, China
2.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China

Funds:Supported by Major Research Instrument Development Projects at the Chinese Academy of Sciences (No. GJJSTD2019003); National Natural Science Foundation of China Youth Fund (No. 61705251); Basic Research Pilot Project of Suzhou (No. SJC2021021); the Scientific Instrument Developing Project of the Chinese Academy of Sciences (No. YJKYYQ20200052)

More Information

Corresponding author:jiahb@sibet.ac.cn
Received Date:29 Apr 2022
Rev Recd Date:31 May 2022

Available Online:21 Jul 2022

Abstract

Abstract

Two-photon microscopy’s ability to maintain good spatial resolution in thick biological tissues has led to its application in in-vivo brain imaging studies soon after its conception. As neural networks have cross-scale multidimensional spatio-temporal properties, two-photon microscopy has developed rapidly and significantly in recent years to meet the demand for in-vivo cross-scale imaging of the brain. This paper firstly introduces the working principle of two-photon microscopy, then reviews the progress of two-photon microscopy from five perspectives: imaging field of view, imaging flux, imaging depth, resolution, miniaturization, and analyzes the difficulties and future challenges of cross-scale two-photon in-vivo microscopic imaging technology.
- in-vivo brain imaging,
- cross-scale,
- two-photon microscopy

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References (70)

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