| Citation: | TAN Tian, SHI Tian-yue, WU Chang-feng, PENG Hong-shang. NIR-II fluorescence confocal imaging based on indirect wavefront shaping[J].Chinese Optics.doi:10.37188/CO.2023-0070
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Optical aberrations caused by the scattering of biological tissues limit the imaging performance of optical systems. We have explored a fluorescence confocal imaging technique that utilizes wavefront shaping indirectly, operating in the near-infrared II range. First, we synthesized a highly efficient fluorescent probe in the near-infrared II range, where reducing the scattering of biological tissue can enable high-contrast biopsy imaging. Second, the study investigate the adaptive optical method, utilizing indirect wavefront measurement. The indirect wavefront shaping technology was applied to the laser scanning confocal system, enabling the measurement and compensation of optical aberrations caused by biological tissues, and obtaining imaging of biological tissues with a high signal-to-noise ratio. Finally, an indirect wavefront shaping-based near-infrared II fluorescence confocal imaging system was deployed and relavant experiments were conducted. The experimental outcomes reveal that the system effectively compensates for the aberrations induced by air plates, scattering media and mouse skull, and increases the final signal intensity to 1.47, 1.95 and 2.85 times, respectively. As a result, the final imaging quality is significantly enhanced.
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