在体跨尺度双光子显微成像技术 -

姓名
邮箱
手机号码
标题
留言内容
验证码

在体跨尺度双光子显微成像技术

doi:10.37188/CO.2022-0086

陈帅^{1, 2,},
任林^{1, 2,},
周镇乔^2,,
李敏^2,,
贾宏博^{1, 2,,}

1.
广西大学物理科学与工程技术学院和脑与智能研究中心, 广西南宁 530004
2.
中国科学院苏州生物医学工程技术研究所, 江苏苏州 215163

基金项目:中国科学院院级重大科研仪器研制项目（No. GJJSTD2019003）；国家自然科学基金青年基金（No. 61705251）；苏州市基础研究试点项目（No. SJC2021021）；中国科学院科研仪器设备研制项目资助（No. YJKYYQ20200052）

详细信息

作者简介:
陈　帅（1994—），男，安徽淮南人，广西大学博士研究生，主要从事双光子显微镜系统的研究。E-mail：2007401031@st.gxu.edu.cn
贾宏博（1983—），吉林省吉林市，现任中国科学院苏州生物医学工程技术研究所技术总监、脑科学仪器创新中心主任研究员，中科院“百人计划——技术英才”入选者，广西大学“君武学者”特聘教授。2004年于北京大学获得学士学位，2006年于巴黎高等师范学校获得物理学硕士学位，2007年于巴黎高等师范学校获得生物学硕士学位，2011年于慕尼黑工业大学获得博士学位。主要专注于生物活体显微成像技术的创新及其在脑科学研究中应用的研究。E-mail：jiahb@sibet.ac.cn

中图分类号:Q6
计量
- 文章访问数:786
- HTML全文浏览量:492
- PDF下载量:508
- 被引次数:0
出版历程
- 收稿日期:2022-04-29
- 修回日期:2022-05-31
- 网络出版日期:2022-07-21

In-vivo across-scales two-photon microscopic imaging technique

CHEN Shuai^{1, 2,},
REN Lin^{1, 2,},
ZHOU Zhen-qiao^2,,
LI Min^2,,
JIA Hong-bo^{1, 2,,}

1.
School of Physical Science and Engineering Technology and Center for Brain and Intelligence Research, Guangxi University, Nanning 530004, China
2.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China

Funds:Supported by Major Research Instrument Development Projects at the Chinese Academy of Sciences (No. GJJSTD2019003); National Natural Science Foundation of China Youth Fund (No. 61705251); Basic Research Pilot Project of Suzhou (No. SJC2021021); the Scientific Instrument Developing Project of the Chinese Academy of Sciences (No. YJKYYQ20200052)

More Information

Corresponding author:jiahb@sibet.ac.cn

摘要

摘要:
双光子显微镜在厚生物组织中依然可以保持良好的空间分辨率，这一优点使得其诞生不久就被应用于在体脑成像研究。而神经网络在时空多个维度均具有跨尺度的特点，为满足脑科学研究中在体跨尺度脑成像的需求，双光子显微镜近年来有了快速且显著的发展。本文首先介绍了双光子显微镜的工作原理，然后在成像视野、成像通量、成像深度、分辨率、微型化5个方面详细综述了双光子显微镜研究的新进展，并深入分析了跨尺度双光子在体显微成像技术的难点及未来挑战。
- 在体脑成像/
- 跨尺度/
- 双光子显微镜
Abstract:
Two-photon microscopy’s ability to maintain good spatial resolution in thick biological tissues has led to its application in in-vivo brain imaging studies soon after its conception. As neural networks have cross-scale multidimensional spatio-temporal properties, two-photon microscopy has developed rapidly and significantly in recent years to meet the demand for in-vivo cross-scale imaging of the brain. This paper firstly introduces the working principle of two-photon microscopy, then reviews the progress of two-photon microscopy from five perspectives: imaging field of view, imaging flux, imaging depth, resolution, miniaturization, and analyzes the difficulties and future challenges of cross-scale two-photon in-vivo microscopic imaging technology.
- in-vivo brain imaging/
- cross-scale/
- two-photon microscopy

HTML全文

图 1（a）单光子和（b）双光子荧光激发原理

Figure 1.Excitation principles of (a) single photon and (b) two-photon fluorescence

下载: 全尺寸图片幻灯片

图 2双光子显微镜结构示意图

Figure 2.Schematic diagram of two-photon microscope structure

下载: 全尺寸图片幻灯片

图 3大尺度成像视野双光子显微镜。（a）多区域实时双光子成像技术^[25]；（b）双扫描系统双光子显微镜^[26]；（c）双扫描区域同步成像双光子显微镜^[28]；（d）多区域随机扫描双光子显微镜^[31]。

Figure 3.Two-photon microscope with large-scale imaging field of view. (a) Multi-area real-time two-photon imaging technology^[25]; (b) dual scanning system two-photon microscope^[26]; (c) two-photon microscope with dual scanning area simultaneous imaging^[28]; (d) two-photon microscope with multi-region follow-on scanning^[31]

下载: 全尺寸图片幻灯片

图 4快速扫描双光子成像技术。（a）光珠双光子显微镜^[37]；（b）多焦点快速扫描双光子显微镜^[38]；（c）快速线扫描双光子显微镜^[39]

Figure 4.Fast scanning two-photon imaging technology. (a) Light beads two-photon microscope^[37]; (b) multi-focus fast scanning two-photon microscope^[38]; (c) fast line scanning two-photon microscope^[39]

下载: 全尺寸图片幻灯片

图 5超深度探测显微成像技术。（a）梯度折射率透镜双光子显微镜^[44]；（b）三光子小鼠神经元成像^[49]；（c）三光子小鼠脑血管成像^[51]

Figure 5.Ultra depth detection microscopic imaging. (a) Gradient refractive index lens two-photon microscope^[44]; (b) three-photon neuron imaging in mice^[49]; (c) three-photon cerebral vascular imaging in mice^[51]

下载: 全尺寸图片幻灯片

图 6超分辨率双光子显微镜。（a）STEM成像原理和实验数据^[58]；（b）SIM结构光生成方法和结构光与均匀光照射探测精度对比^[60]

Figure 6.Super-resolution two-photon microscope. (a) STEM imaging principles and experimental data^[58]; (b) SIM structured light generation method and comparison of structured light and uniform light irradiation detection accuracy^[60]

下载: 全尺寸图片幻灯片

图 7超分辨率双光子显微镜实验结果^[65-66]

Figure 7.Experimental results of super-resolution two-photon microscope^[65-66]

下载: 全尺寸图片幻灯片

表 1双光子显微镜在多个尺度方向的性能提升进展现状

Table 1.Progress in performance improvement of two-photon microscopy in multiple scale directions

提升的尺度方向	提升方法	结果	相关文献
成像视野	1. 使用两套或多套独立扫描探测系统； 2. 自制大视野介观物镜，双焦点扫描，脉冲延时与时分复用； 3. 自制大口径介观物镜，共振镜串联大孔径振镜，随机扫描； 4. 使用多层阵列复合物镜结构，二次聚焦放大，多光轴耦合。	将传统显微镜的成像视野直径由不到1 mm提升至12 mm。	[25−33]
成像通量	1. 光珠双光子荧光显微镜，轴向多焦点扫描； 2. 微透镜阵列将光束分束，平面多焦点扫描； 3. 线扫描，通过压缩传感算法反解出二维荧光图像。	成像通量由百万量级提高至亿量级。图像帧率达到kHz量级。	[35−39]
成像深度	1. 使用更低能量（波长1300 nm）的光子，结合自适应光学，三光子激发； 2. 配合使用梯度折射率透镜，任意深度探测。	成像深度由传统双光子0.7 mm提升至2.1 mm（三光子，无外源装置侵入）或任意深度（配合植入器件）。	[44−46]、[49−51]
成像分辨率	1. 受激辐射耗尽双光子荧光显微镜； 2. 结构光双光子荧光显微镜。	将双光子的成像分辨率由~500 nm提升至80 nm。	[54]、[57−59]、[60−62]
微型化	1. 光纤传导激发光，MEMS、微型物镜等器件。	重量由数十 kg减少到 ~3 g；被观测动物在实验过程中可以自由移动。	[65−68]

下载: 导出CSV

参考文献 (70)

[1]	DENK W, STRICKLER J H, WEBB W W. Two-photon laser scanning fluorescence microscopy[J].Science, 1990, 248(4951): 73-76.doi:10.1126/science.2321027
[2]	JUVEKAR V, LEE H W, KIM H M. Two-photon fluorescent probes for detecting enzyme activities in live tissues[J].ACS Applied Bio Materials, 2021, 4(4): 2957-2973.doi:10.1021/acsabm.1c00063
[3]	ZHANG K Y, CHEN SH, SUN H M,et al.In vivotwo-photon microscopy reveals the contribution of Sox9⁺cell to kidney regeneration in a mouse model with extracellular vesicle treatment[J].Journal of Biological Chemistry, 2020, 295(34): 12203-12213.doi:10.1074/jbc.RA120.012732
[4]	ROTH R H, CUDMORE R H, TAN H L,et al. Cortical synaptic AMPA receptor plasticity during motor learning[J].Neuron, 2020, 105(5): 895-908.E5.doi:10.1016/j.neuron.2019.12.005
[5]	YUSTE R, DENK W. Dendritic spines as basic functional units of neuronal integration[J].Nature, 1995, 375(6533): 682-684.doi:10.1038/375682a0
[6]	DENK W, SVOBODA K. Photon upmanship: why multiphoton imaging is more than a gimmick[J].Neuron, 1997, 18(3): 351-357.doi:10.1016/S0896-6273(00)81237-4
[7]	LICHTMAN J W, DENK W. The big and the small: challenges of imaging the brain’s circuits[J].Science, 2011, 334(6056): 618-623.doi:10.1126/science.1209168
[8]	KANDEL E R.Principles of Neural Science[M]. New York: McGraw-Hill Medical, 2012.
[9]	PODOR B, HU Y L, OHKURA M,et al. Comparison of genetically encoded calcium indicators for monitoring action potentials in mammalian brain by two-photon excitation fluorescence microscopy[J].Neurophotonics, 2015, 2(2): 021014.doi:10.1117/1.NPh.2.2.021014
[10]	VILLETTE V, CHAVARHA M, DIMOV I K,et al. Ultrafast two-photon imaging of a high-gain voltage indicator in awake behaving mice[J].Cell, 2019, 179(7): 1590-1608.E23.doi:10.1016/j.cell.2019.11.004
[11]	DÜRST C D, OERTNER T G.Imaging Synaptic Glutamate Release with Two-Photon Microscopy in Organotypic Slice Cultures[M]//DAHLMANNS J, DAHLMANNS M. Synaptic Vesicles: Methods and Protocols. New York: Humana, 2022: 205-219.
[12]	KOEKKOEK L L, SLOMP M, CASTEL J,et al. Disruption of lateral hypothalamic calorie detection by a free choice high fat diet[J].FASEB Journal, 2021, 35(9): e21804.
[13]	ROY R K, ALTHAMMER F, SEYMOUR A J,et al. Inverse neurovascular coupling contributes to positive feedback excitation of vasopressin neurons during a systemic homeostatic challenge[J].Cell Reports, 2021, 37(5): 109925.doi:10.1016/j.celrep.2021.109925
[14]	LICHTMAN J W, CONCHELLO J A. Fluorescence microscopy[J].Nature Methods, 2005, 2(12): 910-919.doi:10.1038/nmeth817
[15]	ZIPFEL W R, WILLIAMS R M, WEBB W W. Nonlinear magic: multiphoton microscopy in the biosciences[J].Nature Biotechnology, 2003, 21(11): 1369-1377.doi:10.1038/nbt899
[16]	XU C, WEBB W W. Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm[J].Journal of the Optical Society of America B, 1996, 13(3): 481-491.doi:10.1364/JOSAB.13.000481
[17]	HELMCHEN F, DENK W. Deep tissue two-photon microscopy[J].Nature Methods, 2005, 2(12): 932-940.doi:10.1038/nmeth818
[18]	SVOBODA K, BLOCK S M. Biological applications of optical forces[J].Annual Review of Biophysics and Biomolecular Structure, 1994, 23: 247-285.doi:10.1146/annurev.bb.23.060194.001335
[19]	SQUIRRELL J M, WOKOSIN D L, WHITE J G,et al. Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability[J].Nature Biotechnology, 1999, 17(8): 763-767.doi:10.1038/11698
[20]	SO P T, DONG C Y, MASTERS B R,et al. Two-photon excitation fluorescence microscopy[J].Annual Review of Biomedical Engineering, 2000, 2: 399.doi:10.1146/annurev.bioeng.2.1.399
[21]	PATTERSON G H, PISTON D W. Photobleaching in two-photon excitation microscopy[J].Biophysical Journal, 2000, 78(4): 2159-2162.doi:10.1016/S0006-3495(00)76762-2
[22]	HOPT A, NEHER E. Highly nonlinear photodamage in two-photon fluorescence microscopy[J].Biophysical Journal, 2001, 80(4): 2029-2036.doi:10.1016/S0006-3495(01)76173-5
[23]	KUCHIBHOTLA K V, GILL J V, LINDSAY G W,et al. Parallel processing by cortical inhibition enables context-dependent behavior[J].Nature Neuroscience, 2017, 20(1): 62-71.doi:10.1038/nn.4436
[24]	STRINGER C, PACHITARIU M, STEINMETZ N,et al. High-dimensional geometry of population responses in visual cortex[J].Nature, 2019, 571(7765): 361-365.doi:10.1038/s41586-019-1346-5
[25]	YANG M K, ZHOU ZH Q, ZHANG J X,et al. MATRIEX imaging: multiarea two-photon real-time in vivo explorer[J].Light:Science&Applications, 2019, 8: 109.
[26]	LECOQ J, SAVALL J, VUČINIĆ D,et al. Visualizing mammalian brain area interactions by dual-axis two-photon calcium imaging[J].Nature Neuroscience, 2014, 17(12): 1825-1829.doi:10.1038/nn.3867
[27]	KIM T H, SCHNITZER M J. Fluorescence imaging of large-scale neural ensemble dynamics[J].Cell, 2022, 185(1): 9-41.doi:10.1016/j.cell.2021.12.007
[28]	STIRMAN J N, SMITH I T, KUDENOV M W,et al. Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain[J].Nature Biotechnology, 2016, 34(8): 857-862.doi:10.1038/nbt.3594
[29]	CLOUGH M, CHEN I A, PARK S W,et al. Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds[J].Nature Communications, 2021, 12(1): 6638.doi:10.1038/s41467-021-26737-3
[30]	YU CH H, STIRMAN J N, YU Y Y,et al. Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry[J].Nature Communications, 2021, 12(1): 6639.doi:10.1038/s41467-021-26736-4
[31]	SOFRONIEW N J, FLICKINGER D, KING J,et al. A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging[J].eLife, 2016, 5: e14472.doi:10.7554/eLife.14472
[32]	YAO J, GAO Y F, YIN Y X,et al. Exploiting the potential of commercial objectives to extend the field of view of two-photon microscopy by adaptive optics[J].Optics Letters, 2022, 47(4): 989-992.doi:10.1364/OL.450973
[33]	姚靖, 吴婷, 叶世蔚, 等. 离轴抛物镜扫描中继系统提升双光子显微成像视场[J]. 生物学报,2020,29(3):217-224.doi:10.3969/j.issn.1007-7146.2020.03.004 YAO J, WU T, YE SH W,et al. Off-axis parabolic mirror afocal scanning system extends the imaging area of two-photon microscopy[J].Acta Laser Biology Sinica, 2020, 29(3): 217-224. (in Chinese)doi:10.3969/j.issn.1007-7146.2020.03.004
[34]	PAPAGIAKOUMOU E, RONZITTI E, EMILIANI V. Scanless two-photon excitation with temporal focusing[J].Nature Methods, 2020, 17(6): 571-581.doi:10.1038/s41592-020-0795-y
[35]	GAO Y F, XIA X Y, LIU L N,et al. Axial gradient excitation accelerates volumetric imaging of two-photon microscopy[J].Photonics Research, 2022, 10(3): 687-696.doi:10.1364/PRJ.441778
[36]	SONG A, CHARLES A S, KOAY S A,et al. Volumetric two-photon imaging of neurons using stereoscopy (vTwINS)[J].Nature Methods, 2017, 14(4): 420-426.doi:10.1038/nmeth.4226
[37]	DEMAS J, MANLEY J, TEJERA F,et al. High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy[J].Nature Methods, 2021, 18(9): 1103-1111.doi:10.1038/s41592-021-01239-8
[38]	ZHANG T, HERNANDEZ O, CHRAPKIEWICZ R,et al. Kilohertz two-photon brain imaging in awake mice[J].Nature Methods, 2019, 16(11): 1119-1122.doi:10.1038/s41592-019-0597-2
[39]	KAZEMIPOUR A, NOVAK O, FLICKINGER D,et al. Kilohertz frame-rate two-photon tomography[J].Nature Methods, 2019, 16(8): 778-786.doi:10.1038/s41592-019-0493-9
[40]	WU J L, LIANG Y J, CHEN SH,et al. Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo[J].Nature Methods, 2020, 17(3): 287-290.doi:10.1038/s41592-020-0762-7
[41]	WU J L, XU Y Q, XU J J,et al. Ultrafast laser-scanning time-stretch imaging at visible wavelengths[J].Light:Science&Applications, 2017, 6(1): e16196.
[42]	KARPF S, RICHE C T, DI CARLO D,et al. Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates[J].Nature Communications, 2020, 11(1): 2062.doi:10.1038/s41467-020-15618-w
[43]	JUNG J C, MEHTA A D, AKSAY E,et al. In vivo mammalian brain imaging using one- and two-photon fluorescence microendoscopy[J].Journal of Neurophysiology, 2004, 92(5): 3121-3133.doi:10.1152/jn.00234.2004
[44]	BOCARSLY M E, JIANG W CH, WANG CH,et al. Minimally invasive microendoscopy system forin vivofunctional imaging of deep nuclei in the mouse brain[J].Biomedical Optics Express, 2015, 6(11): 4546-4556.doi:10.1364/BOE.6.004546
[45]	ANTONINI A, SATTIN A, MORONI M,et al. Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness[J].eLife, 2020, 9: e58882.doi:10.7554/eLife.58882
[46]	QIN ZH Y, CHEN C P, HE S C,et al. Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes[J].Science Advances, 2020, 6(40): eabc6521.doi:10.1126/sciadv.abc6521
[47]	OHEIM M, BEAUREPAIRE E, CHAIGNEAU E,et al. Two-photon microscopy in brain tissue: parameters influencing the imaging depth[J].Journal of Neuroscience Methods, 2001, 111(1): 29-37.doi:10.1016/S0165-0270(01)00438-1
[48]	THEER P, HASAN M T, DENK W. Two-photon imaging to a depth of 1000 µm in living brains by use of a Ti∶Al₂O₃regenerative amplifier[J].Optics Letters, 2003, 28(12): 1022-1024.doi:10.1364/OL.28.001022
[49]	STREICH L, BOFFI J C, WANG L,et al. High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy[J].Nature Methods, 2021, 18(10): 1253-1258.doi:10.1038/s41592-021-01257-6
[50]	JI N. Adaptive optical fluorescence microscopy[J].Nature Methods, 2017, 14(4): 374-380.doi:10.1038/nmeth.4218
[51]	LIU H J, DENG X Q, TONG SH,et al. In vivo deep-brain structural and hemodynamic multiphoton microscopy enabled by quantum dots[J].Nano Letters, 2019, 19(8): 5260-5265.doi:10.1021/acs.nanolett.9b01708
[52]	INAVALLI V V G K, LENZ M O, BUTLER C,et al. A super-resolution platform for correlative live single-molecule imaging and STED microscopy[J].Nature Methods, 2019, 16(12): 1263-1268.doi:10.1038/s41592-019-0611-8
[53]	FÜRSTENBERG A, HEILEMANN M. Single-molecule localization microscopy-near-molecular spatial resolution in light microscopy with photoswitchable fluorophores[J].Physical Chemistry Chemical Physics, 2013, 15(36): 14919-14930.doi:10.1039/c3cp52289j
[54]	REGO E H, SHAO L.Practical Structured Illumination Microscopy[M]//VERVEER P J. Advanced Fluorescence Microscopy: Methods and Protocols. New York: Humana Press, 2015: 175-192.
[55]	SCHRADER M, MEINECKE F, BAHLMANN K,et al. Monitoring the excited state of a fluorophore in a microscope by stimulated emission[J].Bioimaging, 1995, 3(4): 147-153.doi:10.1002/1361-6374(199512)3:4<147::AID-BIO1>3.0.CO;2-H
[56]	RITTWEGER E, HAN K Y, IRVINE S E,et al. STED microscopy reveals crystal colour centres with nanometric resolution[J].Nature Photonics, 2009, 3(3): 144-147.doi:10.1038/nphoton.2009.2
[57]	BIANCHINI P, DIASPRO A. Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam[J].Journal of Microscopy, 2012, 245(3): 225-228.doi:10.1111/j.1365-2818.2011.03577.x
[58]	BIANCHINI P, HARKE B, GALIANI S,et al. Single-wavelength two-photon excitation–stimulated emission depletion (SW2PE-STED) superresolution imaging[J].Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(17): 6390-6393.doi:10.1073/pnas.1119129109
[59]	LI Y, LIU SH J, SUN D Q,et al. Single-layer multitasking vortex-metalens for ultra-compact two-photon excitation STED endomicroscopy imaging[J].Optics Express, 2021, 29(3): 3795-3807.doi:10.1364/OE.416698
[60]	LI Z W, HOU J, SUO J L,et al. Contrast and resolution enhanced optical sectioning in scattering tissue using line-scanning two-photon structured illumination microscopy[J].Optics Express, 2017, 25(25): 32010-32020.doi:10.1364/OE.25.032010
[61]	URBAN B E, YI J, CHEN S Y,et al. Super-resolution two-photon microscopy via scanning patterned illumination[J].Physical Review E, 2015, 91(4): 042703.
[62]	ZHENG W, WU Y C, WINTER P,et al. Adaptive optics improves multiphoton super-resolution imaging[J].Nature Methods, 2017, 14(9): 869-872.doi:10.1038/nmeth.4337
[63]	SUN SH Y, HE M F, ZHANG ZH M,et al. Enhancing the axial resolution of two-photon imaging[J].Applied Optics, 2019, 58(18): 4892-4897.doi:10.1364/AO.58.004892
[64]	YE SH W, YIN Y X, YAO J,et al. Axial resolution improvement of two-photon microscopy by multi-frame reconstruction and adaptive optics[J].Biomedical Optics Express, 2020, 11(11): 6634-6648.doi:10.1364/BOE.409651
[65]	ZONG W J, WU R L, LI M L,et al. Fast high-resolution miniature two-photon microscopy for brain imaging in freely behaving mice[J].Nature Methods, 2017, 14(7): 713-719.doi:10.1038/nmeth.4305
[66]	OZBAY B N, FUTIA G L, MA M,et al. Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning[J].Scientific Reports, 2018, 8(1): 8108.doi:10.1038/s41598-018-26326-3
[67]	HENDRIKS B H W, KUIPER S, VAN AS M A J,et al. Electrowetting-based variable-focus lens for miniature systems[J].Optical Review, 2005, 12(3): 255-259.doi:10.1007/s10043-005-0255-z
[68]	ZONG W J, OBENHAUS H A, SKYTØEN E R,et al. Large-scale two-photon calcium imaging in freely moving mice[J].Cell, 2022, 185(7): 1240-1256.E30.doi:10.1016/j.cell.2022.02.017
[69]	LOTT G E, MARCINIAK M A, BURKE J H. Three-dimensional imaging of trapped cold atoms with a light field microscope[J].Applied Optics, 2017, 56(31): 8738-8745.doi:10.1364/AO.56.008738
[70]	STEFANOIU A, SCROFANI G, SAAVEDRA G,et al. What about computational super-resolution in fluorescence Fourier light field microscopy?[J].Optics Express, 2020, 28(11): 16554-16568.doi:10.1364/OE.391189