Longitudinal chromatic aberration compensation method for dual-wavelength retinal imaging adaptive optics systems
doi:10.37188/CO.EN.2021-0009
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摘要:双波长视网膜成像自适应光学系统非常适用于视网膜微血管的高对比度和高分辨率成像。本文重点研究了双波长自适应系统的轴向色差补偿问题。首先对轴向色差进行了测量，对实测波前进行了分析，并给出任意波前轴向色差补偿法。自适应校正实验结果显示，色差补偿后，波前均方根误差减小到0.16 λ（λ=589 nm），视网膜微血管分辨率提高到6 μm。这项工作可用于视网膜成像的临床应用。Abstract:Dual-wavelength retinal imaging adaptive optics systems are suitable for high contrast and resolution imaging of retinal capillaries. The compensation of the Longitudinal Chromatic Aberrations (LCAs) in dual-wavelength adaptive systems is researched. The LCA is measured, the measured wavefronts are analyzed, and the arbitrary wavefront LCA compensation method is given. An adaptive correction experiment is carried out and the experimental results indicate that the root mean square error of the wavefront is reduced to 0.16 λ (λ=589 nm) and the retinal capillary resolution is improved to 6 μm. This work may be used for the clinical applications of retinal imaging.
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Figure 1.Illustration of the optical setup for LCA measurement: two lasers are used with the wavelength of 589 nm and 808 nm
Figure 3.Measured wavefronts of subject A. (a) Wavelength of 589 nm; (b) wavelength of 808 nm; (c) LCA; (d) LCA without defocus
Figure 5.Wavefronts of LCA at different times for subject A. (a) Start; (b) 5 minutes later; (c) 1 hour later; (d) 10 hours later; (e) 15 hours later; (f) 24 hours later; (g) 30 hours later; (h) 36 hours later
Figure 7.Calculated compensation of LCA for subject A at different times while the first wavefront of Fig. 5 is chosen as the arbitrary wavefront. The mean value of LCA is 0.16 λ with the standard deviation of 0.017. (a) Start; (b) 5 minutes later; (c) 1 hour later; (d) 10 hours later; (e) 15 hours later; (f) 24 hours later; (g) 30 hours later; (h) 36 hours later
Figure 8.Compensation of LCA for subject A at different times. The mean value of the arbitrary wavefront is 0.158±0.011 λ.
Figure 9.Compensation of LCA for different subjects. The mean values of the arbitrary wavefront is 0.166±0.017 λ.
Figure 10.Optical layout for the retinal imaging AOS: L1-L13, Lens 1- Lens 13; PBS, polarizing beam splitter; BS, beam splitter; an 808 nm laser is used for wavefront detection, tracing and positioning the capillary; a 589 nm laser is used for high contrast imaging of the capillary; the collimated beam comes from the illumination system and is reflected into the eye, and then reflected again out from the eye by the retina; this reflected light is detected and corrected by the adaptive optics system and imaged with an imaging camera; the pupil position is observed by the pupil monitoring system and the eye is fixed with the target staring system
Figure 12.Experiment results of adaptive correction and LCA compensation for subjects A and C. (a) Measured aberration at 808 nm for subject A; (b) wavefront of LCA for subject A; (c) image of retinal capillary without LCA compensation for subject A; (d) image of retinal capillary after LCA compensation for subject A; (e) measured aberration at 808 nm for subject C; (f) wavefront of LCA for subject C; (g) image of retinal capillary without LCA compensation for subject C; (h) image of retinal capillary after LCA compensation for subject C
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