Abstract:Both fluorescence lifetime and time resolved fluorescence spectra are very important in time-resolved fluoroimmunoassay. This article describes a CPLD-based high-speed data acquisition system.In this design,the fluorescence lifetime from a few hundred nanoseconds to more than a dozen milliseconds can be measured. VHDL language had been used in this design of CPLD’s internal logic. There are 13 sampling frequencys can be changed from one to another.The number of sampling can be changed from 1 to 8192 ,also sampling time and delay time can be set arbitrarily, Measurement indicates that the system does well in the measurement of fluorescence lifetime and time-resolved fluoroimmunoassay.