Jun. 2018

Article Contents

Chinese Optics> 2018> 11(3): 363-376.

ZHENG Ai-xian, ZHANG Xiao-long, LIU Xiao-long. Application in nucleic acid functionalized nanoprobe in cellular fluorescence imaging[J]. Chinese Optics, 2018, 11(3): 363-376. doi: 10.3788/CO.20181103.0363

Citation:

ZHENG Ai-xian, ZHANG Xiao-long, LIU Xiao-long. Application in nucleic acid functionalized nanoprobe in cellular fluorescence imaging[J].Chinese Optics, 2018, 11(3): 363-376.doi:10.3788/CO.20181103.0363

Citation:

ZHENG Ai-xian, ZHANG Xiao-long, LIU Xiao-long. Application in nucleic acid functionalized nanoprobe in cellular fluorescence imaging[J].Chinese Optics, 2018, 11(3): 363-376.doi:10.3788/CO.20181103.0363

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Application in nucleic acid functionalized nanoprobe in cellular fluorescence imaging

doi:10.3788/CO.20181103.0363

The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, China

Funds:

National Natural Science Foundation of China21705022

National Natural Science Foundation of China21605021

National Natural Science Foundation of China61575044

More Information

Corresponding author:LIU Xiao-long, E-mail:xiaoloong.liu@gmail.com
Received Date:26 Jan 2018
Rev Recd Date:28 Feb 2018
Publish Date:01 Jun 2018

Abstract

Abstract

Nucleic acid is a substance that carries genetic information. It exists either in nature or can be synthesized by established techniques. Nucleic acid sequences with special functions, such as aptamers and DNAzymes, can also be selected using in vitro techniques. Nucleic acids hybridize according to the principle of Watson-Crick base pairing, and have strong specificity. Whether through sequence design or in vitro screening, nucleic acid probes play an important role in the analysis and imaging applications of biomarkers. In addition, nanomaterials can be used to construct nucleic acid functionalized nanoprobes, which can protect the loaded nucleic acids from being degraded by nucleases and can enter cells without the aid of the transfection reagents. Therefore, nanomaterials have great advantages in the application of cell fluorescence imaging. In order to solve the problem of low intracellular biomarker content and thus difficult to detect, a variety of imaging signal amplification methods suitable for the cellular level have been developed to achieve highly sensitive imaging of low-abundance biomarkers. In this paper, the application of nucleic acid functionalized nanoprobes in cellular fluorescence imaging, including antisense oligonucleotide functionalized nanoprobes, aptamer functionalized nanoprobes and DNAzyme functionalized nanoprobes and that in imaging signal amplification are reviewed.
- nucleic acid,
- nanoprobe,
- antisense oligonucleotide,
- aptamer,
- DNAzyme,
- fluorescence imaging

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References (50)

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