美洛昔康与溶菌酶作用机制的光谱分析及理论模建研究

程旭; 刘保生; 张红彩

doi:10.3788/CO.20201302.0354

Volume 13 Issue 2

Apr. 2020

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Chinese Optics > 2020 > 13(2): 354-362.

CHENG Xu, LIU Bao-sheng, ZHANG Hong-cai. Spectral analysis and theoretical modeling of the working mechanism of meloxicam and lysozyme[J]. Chinese Optics, 2020, 13(2): 354-362. doi: 10.3788/CO.20201302.0354

Citation:

CHENG Xu, LIU Bao-sheng, ZHANG Hong-cai. Spectral analysis and theoretical modeling of the working mechanism of meloxicam and lysozyme[J]. Chinese Optics, 2020, 13(2): 354-362. doi: 10.3788/CO.20201302.0354

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Spectral analysis and theoretical modeling of the working mechanism of meloxicam and lysozyme

doi: 10.3788/CO.20201302.0354

CHENG Xu^{1, 2, 3
,},
LIU Bao-sheng^{1, 2, 3
,
,},
ZHANG Hong-cai^{1, 2, 3}

1.
College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China
2.
Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China
3.
National Chemistry Experimental Teaching Demonstration Center, Baoding 071002, China

Funds:

National Natural Science Foundation of China 21375032

More Information

Corresponding author: LIU Bao-sheng, E-mail:lbs@hbu.edu.cn
Received Date: 29 Mar 2019
Rev Recd Date: 30 Apr 2019
Publish Date: 01 Apr 2020

Abstract

Abstract

In order to explore the active mechanisms of meloxicam and lysozyme, the interaction between the molecules of rheumatoid arthritis drugs, meloxicam and lysozyme was studied using fluorescence spectroscopy, synchronous fluorescence spectroscopy and theoretical modeling analysis under the pH=7.40 experimental conditions. The results showed that meloxicam was able to effectively quench the endogenous fluorescence of lysozyme, forming a 1:1 complex and changing the conformation of lysozyme. Thermodynamic results indicated that the main type of meloxicam-lysozyme system was a hydrophobic interaction. The results of theoretical modeling indicated that the system had hydrogen bonds in addition to hydrophobic interactions and that meloxicam was surrounded by the active amino acid residues Glu35 and Asp52 from the lysozyme, which changed the microenvironment of amino acid residues at the active center of the lysozyme. When a patient took meloxicam 15 mg, the protein binding rate of meloxicam to lysozyme W(B) was 3.71%~8.79%, indicating that the combination of meloxicam and lysozyme has little effect on anti-inflammatory and antibacterial function of lysozyme itself, and the system drug binding rate W(Q) was 1.08%~1.14%, indicating that the combination of lysozyme and meloxicam does not affect the efficacy of meloxicam. This study theoretically proved that lysozyme does not have a serious effect on its own function or the efficacy of meloxicam after it is combined with meloxicam in plasma.
- meloxicam,
- lysozyme,
- fluorescence analysis,
- theoretical modeling,
- binding rate

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References(29)

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